Skip to main content

Table 1 In vitro and vivo evaluation of toxin neutralizing antibody following immunization of mice with DNA vaccine

From: A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile

Vaccinea

Dose

Toxin neutralizing titer ED50b

Toxin challenge surivalc

Anti-TcdA

Anti-TcdB

TcdA

TcdB

TcdA + B

p

50 μg

0

0

0

0

0

pTA

100 ± 30.15

0

90 %

0

0

pTB

0

1133 ± 327.9

10 %

100 %

0

pTAB

140 ± 27.63

1200 ± 294.4

100 %

100 %

80 %

  1. aMice received three immunizations (day 0, day 14 and day 28)
  2. bSera were obtained 7 days following the third immunization. The data are expressed as geometric mean toxin neutralizing titer ± Standard Error of the Mean (SEM) of 10 mice per group
  3. cBalb/C mice (10 mice/group) were challenged i.p with 50 ng TcdA or/and 25 ng TcdB 10 days following the second boost of DNA immunization