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Table 1 In vitro and vivo evaluation of toxin neutralizing antibody following immunization of mice with DNA vaccine

From: A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile

Vaccinea Dose Toxin neutralizing titer ED50b Toxin challenge surivalc
Anti-TcdA Anti-TcdB TcdA TcdB TcdA + B
p 50 μg 0 0 0 0 0
pTA 100 ± 30.15 0 90 % 0 0
pTB 0 1133 ± 327.9 10 % 100 % 0
pTAB 140 ± 27.63 1200 ± 294.4 100 % 100 % 80 %
  1. aMice received three immunizations (day 0, day 14 and day 28)
  2. bSera were obtained 7 days following the third immunization. The data are expressed as geometric mean toxin neutralizing titer ± Standard Error of the Mean (SEM) of 10 mice per group
  3. cBalb/C mice (10 mice/group) were challenged i.p with 50 ng TcdA or/and 25 ng TcdB 10 days following the second boost of DNA immunization