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Table 3 Protective efficacy of 3DIII + AD and the “Tick-E-Vac” vaccine on mice upon standard and prime-boost schemes

From: Recombinant domains III of Tick-Borne Encephalitis Virus envelope protein in combination with dextran and CpGs induce immune response and partial protectiveness against TBE virus infection in mice

Preparation

N mice

Day 0 1st dose

Preparation

Day 7 2nd dose

Day 14 3d dose

Day 21b

Day 42 N mice

ELISA

PRNT50

TBEV

Vas

PRNT50

TBEV

Abs

PRNT50

TBEV

Sof

Survived

Healthy

Standard scheme

 3DIII + AD

10

+a

3DIII + AD

+

+

1:4000

<1:10

1:50

<1:10

5c

0

Prime-boost scheme

 Tick-E-Vac

10

+

3DIII + AD

+

+

1:3000

1:20

1:250

1:50

10c

6

Reference scheme

 Tick-E-Vac

10

Tick-E-Vac

+

+

1:1500

1:80

1:100

1:250

10c

10

Control

 Saline

10

+

Saline

+

+

<1:500

<1:10

<1:10

<1:10

0

0

  1. Mice were challenged with the TBEV strain Vasilchenko (Vas). Antibody titers against TBEV strains Vasilchenko, Absettarov (Abs) and Sofjin (Sof) were measured
  2. a+ mice were vaccinated; – mice were not vaccinated
  3. bmice were intraperitoneally (i/p) challenged with 200LD50 TBEV strain Vasilchenko
  4. cp < 0.05 compared to the control group (exact Fisher test)