Recombinant domains III of Tick-Borne Encephalitis Virus envelope protein in combination with dextran and CpGs induce immune response and partial protectiveness against TBE virus infection in mice

Table 2 Antibody titers in sera of mice, immunized with studied preparations and the “Tick-E-Vac”

Preparation	ELISA^a	PRNT50 TBEV strain (subtype)
Preparation	ELISA^a	Vasilchenko (Siberian)	Sofjin (Far-Eastern)	Absettarov (European)
1 week after the 1st immunization
3DIII + Dex	1:10	1:10	1:20	<1:10
3DIII + AD	<1:10	<1:10	1:20	<1:10
Tick-E-Vac	<1:10	<1:10	1:10	<1:10
1 week after the 2nd immunization
3DIII + Dex	1:70	<1:10	<1:10	<1:10
3DIII + AD	1:500	<1:10	1:30	1:30
Tick-E-Vac	n/s^b	1:200	1:250	1:70
1 week after the 3d immunization for 3DIII + Dex and 3DIII + AD; 2 weeks after the 2nd immunization for Tick-E-Vac
3DIII + Dex	1:3000	1:10	1:25	<1:10
3DIII + AD	1:2500	1:20	1:20	<1:10
Tick-E-Vac	n/s	1:20	1:170	1:30
2 weeks after the 3d immunization for 3DIII + Dex and 3DIII + AD; 3 weeks after the 2nd immunization for Tick-E-Vac
3DIII + Dex	1:4000	1:10	1:20	<1:10
3DIII + AD	1:16000	1:20	1:40	<1:10
Tick-E-Vac	n/s	1:20	1:90	1:30

^aELISA was performed by the scheme 3) (see Materials and Methods)
^bn/s – non-specific interaction. Samples of sera of mice immunized with “Tick-E-Vac” showed high optical density in ELISA with both viral AG and negative control AG, thus the interaction was not determined

ISSN: 1471-2334