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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Recombinant domains III of Tick-Borne Encephalitis Virus envelope protein in combination with dextran and CpGs induce immune response and partial protectiveness against TBE virus infection in mice

Fig. 2

Scheme of recombinant plasmids and analysis of recombinant genes’ expression and proteins purification in 12 % SDS-PAGE (Laemmli, 1970). a. Recombinant plasmids pDBD2-DIII. b, c. Coomassie blue-stained 12 % SDS/PAGE gels of cell extracts (b) and purified proteins (c). Target proteins are marked by black arrows. b. Lanes 1, 3, 6, 9 – extracts of bacterial cells before IPTG induction: E. coli M15 [pRep4], E. coli M15 [pRep4, pDBD2-D3S], E. coli M15 [pRep4, pDBD2-D3E], E. coli M15 [pRep4, pDBD2-D3F]. Lanes 4, 5 – cell extracts of E. coli M15 [pRep4, pDBD2-D3S], 7, 8 – of E. coli M15 [pRep4, pDBD2-D3E], 10 – of E. coli M15 [pRep4, pDBD2-D3F] after 2 h IPTG induction. Lane 11 - page ruler unstained molecular mass marker («Fermentas», Lithuania). c. Coomassie blue-stained 12 % SDS/PAGE gels of purified recombinant proteins DBD2-D3S (line 1), DBD2-D3E (line 2) and DBD2-D3F (line 3). Line 4 – molecular mass marker Thermo Scientific PageRuler Unstained Low Range Protein Ladder Part No. 26632

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