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Table 3 Antibody-dependent enhancement (ADE) activity against four dengue virus (DENV) serotypes in post-Japanese encephalitis (JE) vaccination serum samples that were diluted 1:10 or left undiluted

From: Japanese encephalitis vaccine-facilitated dengue virus infection-enhancement antibody in adults

Sample no. (post-JE vaccination)

(n = 35)c

Fold enhancement to DENVa

ADE activity to number of DENV serotypes

DENV-1

DENV-2

DENV-3

DENV-4

Undiluted

1:10-dilution

Undiluted

1:10-dilution

Undiluted

1:10-dilution

Undiluted

1:10-dilution

Undiluted

1:10-dilution

3

–b

–

–

–

–

–

3.1

2.5

1

1

9

–

–

–

–

–

–

2.2

1.9

0

1

11

4.5 a

2.3

3.2

4.0

4.1 a

2.9

2.3

2.7

3

4

13

13.0

2.8

4.0

2.8

5.9

2.1

4.5

2.8

4

3

19

–

–

–

–

4.4

2.6

–

–

1

1

202

14.5

2.6

2.8

3.6

5.3

2.5

3.3

2.4

4

4

211

–

–

4.2

2.6

4.3

3.5

4.9

2.8

3

3

22

3.5

2.0

–

–

–

–

–

–

1

1

313

2.5

3.0

6.6

4.8

2.7

3.2

4.7

2.4

2

4

321

–

–

–

–

2.5

2.5

–

–

0

1

33

6.0

2.5

2.5

3.8

4.1

3.9

4.4

2.2

4

4

43

–

–

5.1

2.8

4.9

4.8

6.6

1.8

3

2

47

–

–

–

–

3.2

3.3

1.6

2.3

0

2

49

–

–

–

–

3.9

2.3

–

–

1

1

512

6.7

2.6

–

–

5.0

2.6

3.3

2.3

3

3

53

–

–

–

–

3.9

2.0

–

–

1

1

54

–

–

–

–

5.0

3.5

–

–

1

1

573

6.7

2.2

5.5

2.0

  

4.5

2.7

3

3

58

4.0

1.9

1.5

3.2

4.1

3.2

3.4

3.8

3

3

593

4.3

4.2

1.2

2.5

4.0

3.9

1.0

3.4

2

4

61

7.3

2.1

–

–

–

–

–

–

1

1

732

–

–

0.4

2.3

4.1

3.5

1.3

3.5

1

3

77

–

–

–

–

6.3

4.0

3.3

1.8

2

1

781

–

–

–

–

2.0

2.2

–

–

0

1

79

2.0

2.2

1.1

3.6

5.0

6.7

3.8

2.7

2

4

80

4.0

2.1

2.5

2.5

5.4

3.2

–

–

3

3

85

–

–

–

–

–

–

6.2

2.6

1

1

861

1.7

2.4

1.0

3.3

2.9

4.6

2.3

2.5

0

4

893

7.0

4.1

4.2

2.0

4.8

5.1

3.3

3.3

4

4

93

–

–

–

–

3.1

3.2

1.9

2.4

0

2

941

–

–

–

–

–

–

5.8

2.6

1

1

951

–

–

–

–

–

–

2.1

2.1

0

1

961

–

–

4.0

2.0

3.8

3.1

3.8

5.2

3

3

100

–

–

–

–

–

–

2.4

2.1

0

1

110

–

–

–

–

4.9

2.8

3.4

2.2

2

2

Total number of samples

12

14

11

16

20

24

17

24

  
  1. aItalics indicates the presence of ADE activity
  2. b–denotes an absence of ADE activity at a 1:10 sample dilution to the tested DENV serotype. Assessments of ADE activity in undiluted samples were not performed in samples that did not demonstrate ADE activity at the 1:10 dilution
  3. cThe numbers in superscript correspond to the number of serotypes to which a sample had ADE activity prior to JE vaccination in this study