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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: Development and evaluation of an in-house single step loop-mediated isothermal amplification (SS-LAMP) assay for the detection of Mycobacterium tuberculosis complex in sputum samples from Moroccan patients

Fig. 4

Limit of detection (LOD) of SS-LAMP. a Limit of Detection (LOD) of the SS-LAMP assay. Tubes 1–7 showed the color change from original orange color to bright green. The tubes contained 106, 105, 104, 103, 102, 10 and 1 copy/reaction of purified reference plasmid (pGEMeasy-IS6110), respectively. NC: Negative control. b Limit of Detection (LOD) of RT-qPCR reaction: Amplification plot shows the normalized fluorescent signal (Rn) versus cycle number. The reaction wells contained duplicates of 106, 105, 104, 103, 102, 10 and 1 copy/reaction of purified reference plasmid (pGEMeasy-IS6110), respectively

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