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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: A high throughput serum bactericidal assay for antibodies to Haemophilus influenzae type b

Fig. 4

Comparison of SBA results with ELISA results. Ten sera with undetectable anti-Hib IgG (closed circles) and 80 sera with ≥ 0.15 μg/mL anti-Hib IgG (open circles) were tested. The anti-Hib IgG concentration (x axis) was compared with the serum bactericidal index, SBI (y axis). The vertical dashed line drawn at a concentration of 0.15 μg/mL anti-Hib IgG indicates the lower limit of the anti-Hib IgG ELISA and the horizontal dashed line at an SBI of 4 indicates the lower limit of the SBA. The vertical solid line drawn at a concentration of 1 μg/mL anti-Hib IgG is shown for comparison. The 10 data points (closed circles) with undetectable anti-Hib antibodies were assigned to have 0.075 μg/mL and 2 SBI for the purpose of statistical calculation

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