Fig. 1

Detection of transcriptive and replicative form of CHPV in human PBMC. The PBMC’s were isolated from the whole blood cells of healthy donors and infected with MOI = 5 CHPV in complete medium (RPMI + 10 % FCS). Cells were harvested at different hours time interval (1, 2, 3 , 8, 12 and 24 h) and total RNA was extracted by Trizol method. The transcriptive form (N gene) and replicative form (NP gene) was detected by RT-PCR. The assay was performed in triplicates