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Table 6 Distribution of Haemophilus influenzae and Haemophilus parainfluenzae biofilm-producers in patients with sarcoidosis and in healthy people

From: Changes in the prevalence and biofilm formation of Haemophilus influenzae and Haemophilus parainfluenzae from the respiratory microbiota of patients with sarcoidosis

Category of biofilm producers OD570 range No. (%) of isolates
Haemophilus influenzae Haemophilus parainfluenzae
Healthy people
   Nasopharynx (n = 1)   Nasopharynx (n = 65)  
Non-producers 0.0 0 (0.0)   8 (12.3)  
Weak 0.01–0.24 1 (100)   15 (23.1)  
Moderate 0.25–0.48 0 (0.0)   3 (4.6)  
Strong 0.49–0.96 0 (0.0)   12 (18.5)  
Very strong ≥0.97 0 (0.0)   27 (41.5)  
Patients with sarcoidosis
   Nasopharynx (n = 4) Sputum (n = 8) Nasopharynx (n = 31) Sputum (n = 26)
Non-producers 0.0 1 (25.0) 3 (37.5) 12 (38.7) 6 (23.1)
Weak 0.01–0.24 3 (75.0) 5 (62.5) 11 (35.5) 9 (34.6)
Moderate 0.25–0.48 0 (0.0) 0 (0.0) 0 (0.0) 3 (11.5)
Strong 0.49–0.96 0 (0.0) 0 (0.0) 1 (3.2) 4 (15.4)
Very strong ≥0.97 0 (0.0) 0 (0.0) 7 (22.6) 4 (15.4)
  1. Distribution of biofilm-producers was detected using the crystal-violet (CV) method. The cut-off OD (ODc; here: 0.1 ± 0.02) was defined as three standard deviations above the mean OD570 of the negative control. The categories of biofilm-producers: 0 - non-producers (OD ≤ ODc); 1, weak (ODc < OD ≤ 2xODc); 2, moderate (2xODc < OD ≤ 4xODc); 3, strong (4xODc < OD ≤ 8xODc); 4, very strong (8xODc < OD) producers, according to [26]