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Table 1 Results from the Experimental trial: Real-time PCR analysis on samples, undergoing different sanitation treatment after experimental contamination by artificial salivary solutions, in presence (Red) and absence (White) of blood

From: Infection control in healthcare settings: perspectives for mfDNA analysis in monitoring sanitation procedures

 

Treatment

No Treatment

Polyphenol 5 %

Superacetic 10 E

Autoclave at 121 °C 30 min

 

Contamination type

 

5 min

5 s diluited 1:10

10 min

5 s diluited 1:10

With cleaning and disinfection step

Without cleaning and disinfection step

Results time PCR (CT mean ± SDa)

Red solution

20.2 ± 2.3

27.6 ± 5

26.6 ± 3

29.5 ± 2.3

26.7 ± 3

NDa

28.7 ± 1.2

White solution

22.4 ± 0.2

28.8 ± 1.6

28.9 ± 2.4

25.9 ± 0.6

25.5 ± 0.5

NDa

28.5 ± 2.4

  1. a The data were expressed as the mean of threshold cycle (CT) of three independent replicates and corresponding standard deviation; ND Not Detected