Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012

Table 1 Primers and probes used in this study

Gene or sequence	Primer/probe sequence (5´–3´)^a	Purpose	Reference
IS481	F: GCCGGATGAACACCCATAAG	Diagnosis	[25]
	R: GCGATCAATTGCTGGACCAT
	P: (FAM)-CGATTGACCTTCCTACGTC-(BHQ1)
IS1001	F: AATTGCTGCAAGCCAACCA	Diagnosis	[25]
	R: CCAGAGCCGTTTGAGTTCGT
	P: (HEX)-ACATAGACCGTCAGCAG-(BHQ2)
IS1002	F: CTAGGTCGAGCCCTTCTTGTTAAC	Diagnosis	[25]
	R: GCGGGCAAGCCACTTGTA
	P:(CY5)CATCGTCCAGTTCTGTTGCATCACCC-(BHQ2)
ptxP	F: AATCGTCCTGCTCAACCGCC	Genotyping	[33]
ptxP	R: GGTATACGGTGGCGGGAGGA	Genotyping	[33]
ptxA	F: CCCCTGCCATGGTGTGATC	Genotyping	[33]
ptxA	R: TCAATTACCGGAGTTGGGCG	Genotyping	[33]
fim2	F: GCGCCGGGCCCTGCATGCAC	Genotyping	[33]
fim2	R: GGGGGGTTGGCGATTTCCAGTTCTC	Genotyping	[33]
fim3	F: GACCTGATATTCTGATGCCG	Genotyping	[33]
fim3	R: AAGGCTTGCCGGTTTTTTTTGG	Genotyping	[33]
Prn	F: CAATGTCACGGTCCAA	Genotyping	[33]
Prn	R: GCAAGGTGATCGACAGGG	Genotyping	[33]

^a F forward primer, R reverse primer; P probes for IS481, IS1001, and IS1002. Fluorophores and quenchers are indicated at the 5´ and 3´ end of each probe, respectively

ISSN: 1471-2334