Fig. 1

Analysis of N. gonorrhoeae drug resistance mutations via hybridisation on a microarray. a Hybridisation microarray configuration. Immobilised oligonucleotide probes targeted the insertion of D345 in the penA gene, L421P mutation in the ponA gene, 2 mutations (V57M and V57L) in the rpsJ gene, S91F, D95N, D95G mutations in the gyrA gene, and 7 mutations (S87N, S87R, S87R2, E91Q, E91G, E91K and E91A) in the parC gene. Elements with wild-type-sequence oligonucleotide probes are depicted with bold circles. M—marker elements with fluorescent label, 0—reference gel elements without oligonucleotides. b Hybridisation analysis of the wild-type N. gonorrhoeae DNA sample. c Hybridisation of a DNA sample containing the following mutations: D345 insertion (penA), L421P (ponA), V57M (rpsJ), and S91F (gyrA). Groups with detected mutations are depicted as boxes