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Table 1 LOD determination for C. trachomatis specific LAMP assaya

From: Combination with antimicrobial peptide lyses improves loop-mediated isothermal amplification based method for Chlamydia trachomatis detection directly in urine sample

 

Copies per reaction

Lower bound 95 %

Upper bound 95 %

Limit of detection in water (0.95 value)

25

21

33

Limit of detection in pooled urine (0.95 value)

70

61

96

  1. aFor LOD determination in water 0, 4, 6, 12, 24, 48 and 120 plasmid copies were applied per reaction in 22 parallels each. For LOD determination in urine 0, 40, 50, 75, 100 and 200 plasmid copies were applied. Each reaction was performed in 20 parallels. Numbers of positive and negative signals visualized on LF strips were analysed by XLSTAT