Table 3 Probe sequences for EMSA
Probes | Sequence (5′–3′) | Biotin | Genes |
|---|---|---|---|
Positive control | PU.1-TP CTGCCTCCTACTTCTCCTTTTCTGC PU.1-BM GCAGAAAAGGAGAAGTAGGTGGCAG | + | CD11b |
Probe A | probe1-TP TTACTTTTGGGGAAGTTGAGTTCAG probe1-BM CTGAACTCAACTTCCCCAAAAGTAA | + | Dectin-1 |
Probe B | probe1-BM CTGAACTCAACTTCCCCAAAAGTAA probe2-TP TGATGAAAGAGGCAGTGTAGCGTAA | + | Dectin-1 |
Probe C | probe3-TP GATTGAACAAGAAAATATGTACAAC probe3-BM GTTGTACATATTTTCTTGTTCAATC | + | Dectin-1 |
Competitor | Paired with each biotin-labeled probe | - | Â |