Fig. 2

Immunofluorescence staining for Dectin-1 and PU.1 expression and location in A. fumigatus conidia-stimulated THP-1 cells. THP-1 cells were untreated (control) or incubated with A. fumigatus conidia for 2, 8, or 12 h. After permeabilization with Triton X-100, the cells were incubated with goat anti-Dectin-1 and rabbit anti-PU.1 antibodies at a 1:100 dilution, stained with Alexa Fluor-568 donkey anti-goat IgG and Alexa Fluor-488 donkey anti-rabbit IgG at 1:200 dilution, counterstained with DAPI, and analyzed by confocal microscopy