Skip to main content

Table 3 Results of HRM curves analysis for rpoB, katG and inhA targets in Mycobacterium tuberculosis

From: High-resolution melting analysis for molecular detection of multidrug resistance tuberculosis in Peruvian isolates

DRUG

PHENOTYPE

TARGET

MUTATION

HRM CURVE ASSAY

DNA SEQUENCING

RIFAMPICIN

Resistant (78)

rpoB

53

S531L (TCG → TTG)

mutant

mutant

14

D516V (GAC → GTC)

mutant

mutant

2

H526D (CAC → GAC)

mutant

mutant

1

Q513L (CAA → CTA)

mutant

mutant

1

Q513P (CAA → CCA)

mutant

mutant

1

M515I (ATG → ATA), D516G (GAC → GGC)

mutant

mutant

1

D516Y (GAC → TAC), N518H (AAC → CAC)

mutant

mutant

1

H526R (CAC → CGC)

mutant

mutant

1

H526Y (CAC → TAC)

mutant

mutant

1

S531F (TCG → TTT)

mutant

mutant

1

S531W (TCG → TGG)

mutant

mutant

1

No mutation

wild

wild

Susceptible (89)

 

87

Not sequenced

wild

-

2

514 Phe (TTC → TTT)

mutant

wild

ISONIAZID

Resistant (78)

katG

66

S315T (AGC → ACC)

mutant

mutant

1

S315T (AGC → ACC), C → T (−15)

mutant

mutant

inhA

10

C → T (−15)

mutant

mutant

katG,inhA

1

No mutation

wild

wild

Susceptible (89)

 

87

Not sequenced

wild

-

 

2

No mutation

wild

wild