Table 1 Sequences of the primers used HRM analysis
Primer | Target | Sequence | Fragment size (bp) | HRM temperature range (°C) |
|---|---|---|---|---|
IS6110-F | TCCGGCCGGTGGTCG | |||
IS6110 | 103 | |||
IS6110-R | CGGCGCTTGTGGGTCAA | |||
rpoB-F | CGCGATCAAGGAGTTCTTC | |||
rpoB | 118 | |||
rpoB-R | TGACAGACCGCCGGGCCC | 80–95 | ||
katG-F | GCTGAGCCAATTCATGGACC | |||
katG | 235 | |||
katG-R | TGACAGACCGCCGGGCCC | |||
inhA-F | GCGGTCACACTTTCGGTAA | |||
Promoter inhA | 180 | |||
inhA-R | GGTGTTCGTCCATACGACCT |