Fig. 1
Delayed treatment of China-made Peramivir enhanced survival, suppressed weight loss and reduced lung virus titer. Mice were administered virus (H1N1pdm2009: 3 LD50; in 50 μL saline) via inhalation to induce acute lung inflammation, and were then treated with Peramivir (60 mg/kg · d, intravenous injection, QD × 5 days) and Oseltamivir (60 mg/kg · d, oral, QD × 5 days). Survival rate (a) and body weight (b) were determined daily for 15 d. Viral titers in the lungs (c) on days 5 and 7 were determined by the cytopathic effect method and the plaque assay. d Lung index of each group on days 5 and 7. The index was determined as the lung weight/final body weight (LW/BW). e Mice were administered virus (influenza B virus: 10 LD50; in 50 μL saline) via inhalation, and were then treated with Peramivir (40 mg/kg · d, intravenous injection, QD × 5 days). Lung index of each group on days 5 and 7 were calculated. f Mice were treated with different doses of Peramivir (40, 60 and 90 mg/kg · d), and the survival rate was determined daily for 15 d. Values are shown as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 Peramivir group versus virus control group; #p < 0.05, ##p < 0.01 and ###p < 0.001 Ositamivir group versus virus control group. Statistical analyses were performed consecutively with ANOVA or the two-tailed Student’s t-test