Fig. 2

Gene expression of inflammatory mediators are augmented in the thymuses of mice infected with P. brasiliensis. Male Balb/c mice (n = 5 mice/group) were injected i.p. with Pb cells. Seven days later, thymuses were processed to mRNA extraction and analyzed for alterations on gene expression of AIRE, IL-7, IL-2, IL-17, IL-10 and TNFα, with GAPDH as housekeeping gene by real-time PCR. (a) Relative mRNA level of IL-2, IL-7 and AIRE in thymus of infected mice. (b) Relative mRNA level of IL-17, TNFα and IL-10. Expression levels of genes were represented as a relative copy numbers by using the method of delta threshold (2^-ΔΔCt). Data was analyzed by Student t test. Values of p ≤ 0,05 (*) were considered statistically significant. Results are expressed by Mean ± SEM. Representative data from three independent experiments with similar results