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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Fast and accurate prediction of positive and negative urine cultures by flow cytometry

Fig. 1

FC bacterial counting in urine samples. Bacteria were stained with SYBR Green and measured by the Accuri C6 as outlined in methods. a-c One representative example of urine bacterial counting. a FSC vs. SSC of bacteria detected in a urine sample (R1). Gate R1 is used in B and C. b FL-1 vs. SSC of an unstained urine sample as a negative control for SYBR Green (SG) staining. R3 gate shows the background staining (0.1 %). c SG vs. SSC of a stained urine sample. R3 gate shows the amount of SG-stained bacteria present in urine sample, which is used to calculate the absolute amount of bacteria per mL urine sample

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