Fig. 1From: Fast and accurate prediction of positive and negative urine cultures by flow cytometryFC bacterial counting in urine samples. Bacteria were stained with SYBR Green and measured by the Accuri C6 as outlined in methods. a-c One representative example of urine bacterial counting. a FSC vs. SSC of bacteria detected in a urine sample (R1). Gate R1 is used in B and C. b FL-1 vs. SSC of an unstained urine sample as a negative control for SYBR Green (SG) staining. R3 gate shows the background staining (0.1 %). c SG vs. SSC of a stained urine sample. R3 gate shows the amount of SG-stained bacteria present in urine sample, which is used to calculate the absolute amount of bacteria per mL urine sampleBack to article page