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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: Immunoregulatory mechanisms in Chagas disease: modulation of apoptosis in T-cell mediated immune responses

Fig. 4

Analyses of cytokine levels and their association with cardiac morbidity expressed by the clinical classification. (a) The analysis of plasma cytokine levels was performed as described in material and methods. The groups evaluated were: NI (n = 15, white box), IND (n = 15, light gray box), and CARD (n = 15, dark gray box). The results were expressed by mean intensity of fluorescence (MIF). Plasma TNF-α levels in NI, IND, and CARD groups and their association with cardiac morbidity. (b, c) Analysis of percentage of CD4+TNFα+ and CD4+TNFα+ T cells in the peripheral blood from chagasic patients, in ex vivo context and after in vitro stimulation with T. cruzi antigens, from patients with distinct clinical forms of Chagas disease. Significant differences (P-value < 0.05) in the charts are identified by connecting lines and the symbol (*) for comparisons between the groups. (d) Correlation analysis between percentage of annexin+ CD4+ and annexin+ CD8+ T cells and TNF-α intracytoplasmic in the IND (n = 15) and CARD (n = 15) groups. Mann–Whitney test was used for comparison and the results were expressed as the median. Correlation analysis were done using the Spearman correlation coefficient, and the results were considered significant with a p-value < 0.05. Significant differences (P-value) are indicated in each graph together with the r values

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