Coxiella burnetii dormancy in a fatal ten-year multisystem dysfunctional illness: case report

BMC Infectious Diseases

Table 1 Quantitative PCR (qPCR) tests for C.b. DNA (IS1111a sequence) with patient BI’s paraffin-embedded tissues were tested in two PCR cyclers – RotorGene 1.7.73 (Corbett Research) and Roche LightCycler 4.05 (4.0.0.23)

Patient’s post mortem specimens	Ct - RotorGene	Ct - LightCycler
Heart	23.34	25.65
Lung	36.37	36.46
Brain	NEG	NEG
Bone marrow	NEG	NEG
Spleen/Liver	25.15	27.08
Lymph nodes	22.47	24.80
Control spleen	NEG	NEG
Control brain	NEG	NEG

The RotorGene Ct values shown are average of duplicate tests and LightCycler values from single tests. Non-template controls (NTC) were placed in between the samples and were negative (Ct >37). Thick sections of the post mortem tissues – for PCR tests - were cut with high level of precaution to minimize possible cross contamination at the Centre for Neurological Diseases (SA Pathology, South Australia)

ISSN: 1471-2334