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Table 1 Antibiotic sensitivity testing results

From: High prevalence of multidrug resistant Enterobacteriaceae isolated from outpatient urine samples but not the hospital environment in Bo, Sierra Leone

ID Identificationa Disk diffusionb E-test ESBLc
ATM CAZ CIP IMP TGC C D G GM K SAM IMP Phenotype CTX-M
Clinical isolates
SL122 Citrobacter freundii R R R S S R R R R R R 0.250 YES YES
SL124 R I R S S R R R R R R 0.380 YES YES
SL127 R I R S I R R R R R R 0.380 YES YES
SL129 S I R S I R R R R I R 0.190 N/D NO
SL150 R R R S I I R R R R R 0.190 YES YES
SL151 R I R S S R R R R I R 0.380 YES YES
SL153 R R R I I R R R R I R 0.250 YES YES
SL154 R R R I S R R R R I R 0.190 N/D NO
SL155 R R R S S R R R R I R 0.250 N/D NO
SL156 R R R I I R I R R R R 0.250 YES YES
SL157 R R R S I R I R R R R 0.250 YES YES
SL170 R I R S S R R R R I R 0.750 YES YES
SL172 R R R S S S R R R R I 0.190 YES YES
SL173 R R R I S R R R R I R 0.380 YES YES
SL177 R R R S S R R R R I R 0.250 YES YES
SL187 R I R S S S I R R I I 0.250 YES YES
SL193 R I R S S R I R R S R 0.250 YES YES
SL194 R R R S S R R R R I R 0.380 N/D NO
SL197 R R R S S S I R R I I 0.250 YES YES
SL200 R R R S I S R R R R R 0.190 YES YES
SL202 R R R S I S R R R R R 0.190 YES YES
SL205 R R R I I S R R R R I 0.190 YES YES
SL160 Enterobacter cloacae S S S S S S S R S S S 0.380 NO NO
SL161 R R I I I R S R R R R 0.250 YES YES
SL167 I I I S S R I R R I R 0.380 YES YES
SL168 R R I I S R I R R I R 2.000 YES YES
SL174 R I I S S R I R R R I 0.380 YES YES
SL175 I I S S S R S R R R R 0.250 YES YES
SL179 R R R S S R I R R R R 0.250 YES YES
SL181 S S S S S R S R R I I 0.380 YES YES
SL182 R R I I S R S R R I R 0.380 YES YES
SL189 S S I S S R S R R I I 0.250 YES YES
SL192 S S I S S R R R R S S 0.380 NO NO
SL195 I I S S S R S R R I I 0.380 YES YES
SL198 R R S S S R S R R I R 0.250 YES YES
SL199 R I I S S R S R R I I 0.380 YES YES
SL203 I I I S S R S R R I I 0.380 YES YES
SL115 Escherichia coli S S S S S S S S S S S 0.190 NO NO
SL121 S S R S S R R R S S I 0.190 NO NO
SL125 S S S S S S S S S S S 0.190 NO NO
SL133 S S S S S S R R S S R 0.190 NO NO
SL137 S S S S S S R R S S R 0.190 NO NO
SL152 S S I S S R R R R S S 0.190 NO NO
SL158 S S R I S R R R S S R 0.190 NO NO
SL159 S S S I S S S S S S S 0.190 NO NO
SL169 S S S S S R R R S S S 0.250 NO NO
SL171 S S S S S R I R S S R 0.250 NO NO
SL176 S S R S S S S R S S I 0.250 NO NO
SL178 R S S S S R I R I S S 0.250 NO NO
SL188 S S S S S R I R S S S 0.250 NO NO
SL166 Escherichia hermannii R R I S S R S R R I R 0.190 YES YES
SL108 Klebsiella pneumoniae S S I S S S S S S S S 0.250 NO NO
SL113 S S I S S R I R R R S 0.250 YES YES
SL116 S S I S S S S S S S S 0.190 NO NO
SL136 S S S S S R S R S S S 0.250 NO NO
SL141 S S S S S S S S S S S 0.250 NO NO
SL162 R R R I I R R R R I R 0.190 YES YES
SL163 R R I I I R R R R S R 0.190 YES YES
SL164 S S I S S R R R R S R 0.190 YES YES
SL165 I I I S S R R R R I R 0.250 YES YES
SL180 S S S S S R S R R I I 0.380 YES YES
SL183 S S S S S R R R S S I 0.250 NO NO
SL185 I I R S S S I R R R I 0.190 YES YES
SL186 I I I S S R R R R S R 0.250 YES YES
SL190 S S R I I R R R S R R 0.250 NO NO
SL196 R R R I I S I R R R I 0.250 YES YES
SL184 Enterobacter sp./Leclercia sp. I I R S S R S R R I I 0.250 YES YES
SL191 I R R I I R S R R I I 0.250 YES YES
SL201 R R R R I R S R R R I 0.250 YES YES
SL204 R R I R I R R R R R I 0.190 YES YES
Environmental isolates
SL512 Enterobacter cloacae S S S I S S R R S S S 0.250 NO NO
SL532 I I S S S R S R R R R N/A YES YES
SL513 Klebsiella pneumoniae R R S S S S S R R I R 0.190 YES YES
SL531 S S S S S S S S S S S 0.190 NO NO
SL522 Pantoea dispersa S S S S S S S S S S S 0.250 NO NO
  1. aConsensus identification based on combination phenotypic (API20E/ID32E) and 16S rRNA and rpoB gene sequencing
  2. bDisk diffusion testing using the following antibiotics: ATM aztreonam, CAZ ceftazidime, CIP ciprofloxacin, IMP imipenem, TGC tigecycline, C chloramphenicol, D doxycycline, G sulfisoxazole, GM gentamicin, K kanamycin, SAM ampicillin/sulbactam. R resistant, I intermediate, and S sensitive phenotypes were determined based on CLSI criteria
  3. cESBL phenotypes: YES – ESBL detected, NO – no ESBL present, N/D – ESBL presence could not be determined based on the E-test or double disk diffusion assay
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