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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Molecular diagnosis of Toxoplasma gondii infection in Libya

Fig. 2

a: PCR amplification of T. gondii SAG2 gene in 1.7 % agarose gel. Lane 1 and 2 = positive HIV whole blood samples. Lanes 3 and 4 = leukaemia whole blood samples. Lane 5 and 6 = aborted woman serum and whole blood respectively. Lane 7 = patient with ocular infection. Ve + = positive control (RH strain). b: Semi-nested PCR amplification of T. gondii SAG2 gene for samples with faint band in the first PCR run. Lanes; 1 and 2 = positive HIV whole blood samples. Lanes 3 and 5 = leukaemia whole blood samples. Lane 4; aborted woman serum sample. Lane 6 = seronegative toxoplasmosis aborted woman whole blood sample. Lane P = positive control (RH strain). Ve- = is negative control (blanked water), M = 100 bp DNA Ladder (BIORON)

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