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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Molecular diagnosis of Toxoplasma gondii infection in Libya

Fig. 1

a: PCR amplification performed with various DNA concentrations. The quantity of DNA is shown above each lane, minimum amount detected was 12 ng. ve + =positive control. b: PCR for detection of T. gondii DNA in whole blood samples spiked with 101, 102 and 103 tachyzoites. Lane N = whole blood without tachyzoites, +ve = positive control (DNA extracted from pure tachyzoites). The bands were quantified by using the software ImageJ. The percentage of the area under the curve was 101 = 12 %, 102 = 25 %, 103 = 30 % and + ve =33 %. -ve = negative control, M = 100-bp DNA ladder (BIORON)

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