Does typing of Chlamydia trachomatis using housekeeping multilocus sequence typing reveal different sexual networks among heterosexuals and men who have sex with men?

Versteeg, Bart; Bruisten, Sylvia M.; van der Ende, Arie; Pannekoek, Yvonne

doi:10.1186/s12879-016-1486-2

Table 1 Primers used for the MLST-7 scheme for Chlamydia trachomatis^a

From: Does typing of Chlamydia trachomatis using housekeeping multilocus sequence typing reveal different sexual networks among heterosexuals and men who have sex with men?

Region	Locus tag^b	Format	Direction	Sequence (5′ to 3′)	5′ position^c	Fragment length (bp)^d
gatA	CT0003	Outer	Forward	GCTTTAGAATTARSARAWGCT	2123	325
		Outer	Reverse	GATCCTCCGGTATCYGATCC	2615
		Inner + Seq	Forward	ATGACGAACAGATTGGAGC	2186
		Inner + Seq	Reverse	GGATTATTGGTAGGATGAA	2532
oppA_3	CT0198	Outer	Forward	ATGCGCAAGATATCAGTGGG	222438	480
		Outer	Reverse	AAAGCTCCRSTWGMTATMGGWAG	223002
		Inner + Seq	Forward	TCCTAGCATTAGCAACTTCT	222469
		Inner + Seq	Reverse	TCTTTCCGTATCTGATGCTGCG	222970
hflX	CT0379	Outer	Forward	GCTTCTARAGTACTTTTAAATG	432737	359
		Outer	Reverse	TATTTRGAAATYTTTKCSAGYCG	432758
		Inner + Seq	Forward	AAGTATGCGGAAGTTTGCG	432768
		Inner + Seq	Reverse	AATCAGGAGGTAGTGGTGGAGG	433106
gidA	CT0498	Outer	Forward	GGAGTCWCTACWAAAGAAGG	577352	389
		Outer	Reverse	TCGTAYTGYACATCRAAAGG	577892
		Inner + Seq	Forward	ACTTCTCTGGGGGACGATT	577449
		Inner + Seq	Reverse	GACCGTTCACATAAACTTCTTG	577856
enoA	CT0587	Outer	Forward	GCAAATACTTTACAGAGACCTT	662222	388
		Outer	Reverse	CGTCACAAATAGGTCGTCTC	662775
		Inner + Seq	Forward	CCTATGATGAATCTKATCAATGG	662288
		Inner + Seq	Reverse	TCTTCTTCGGCTAGCCCATCT	662698
hemN	CT0746	Outer	Forward	GAATCTTGCCTTTCACAGTTGC	867674	448
		Outer	Reverse	ACTTCCACATCCCATTCTGC	868584
		Inner + Seq	Forward	AGATCTTCTTCWGGRGGWAGAGA	867799
		Inner + Seq	Reverse	TTCYTTCAKAACSTAGGTTTT	868269
fumC	CT0855	Outer	Forward	ATTAAAAAATGTGCTGCT	1004691	451
		Outer	Reverse	CCTTCAGGAACATTYAACCC	1005242
		Inner + Seq	Forward	ATTAAAAAATGTGCTGCT	1004691
		Inner + Seq	Reverse	CCGCTCTAAACAATTATGCAACTG	1005159

^aThe primers given in boldface were the original MLST primers taken from Pannekoek et al. [9]. All other primers were newly designed. Nested PCRs were performed for each region. The primers used for the outer PCRs are indicated with Outer, those used for the inner PCRs are indicated with Inner, and those used for sequencing are indicated with Seq
^bLocus tags are given relative to the sequence of reference strain D/UW-3/CX (GenBank accession no. AE001273)
^cPositions are given in base pairs relative to the sequence of reference strain D/UW-3/CX (GenBank accession no. AE001273)
^dFragment length is the number of base pairs sequenced with the primers excluded

Back to article page

ISSN: 1471-2334

Contact us

Submission enquiries: bmcinfectiousdiseases@biomedcentral.com
General enquiries: ORSupport@springernature.com

BMC Infectious Diseases

Contact us