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Table 2 Sequences of primers used in nested multiplex PCR for the detection of Mycobacterium spp.

From: Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments

Genes

Primers

Sequence (5’-3’)

Size (bp)

Reference (Primers)

nMTP40 a

MTB2F

CGTTCGGGATGCACTGCG

342

In this study

 

MTB2R

CACCCGGCGAATTCGTCAC

  

nIS6110 b

MTBC2F

CGATCGCCCCATCGACCTACT

500

In this study

 

MTBC2R

GGTCGAGTACGCCTTCTTGT

  

n32kD α-antigen c

NTM2F

CACCCGCAGTTCATCTA

413

In this study

 

NTM2R

CGTTGTAGGCGTCCTGG

  
  1. n Primers used in nested PCR
  2. aspecific for Mycobacterium tuberculosis
  3. bspecific for Mycobacterium tuberculosis complex
  4. cspecific for non-tuberculous mycobacterium
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BMC Infectious Diseases

ISSN: 1471-2334

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