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Table 1 Sequences of primers used in the detection of Mycobacterium spp. in single step multiplex PCR assay

From: Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments

Genes

Primers

Sequence (5’-3’)

Size (bp)

Reference

MTP40 a

MTB F

CGGCAACGCGCCGTCGGTGG

396

Herrera E. A. 1996 [15]

 

MTB R

CCCCCCACGGCACCGCCGGG

  

IS6110 b

MTBC F

CGGAGACGGTGCGTAAGTGG

984

Wojciech, 1992 [39]

 

MTBC R

GATGGACCGCCAGGGCTTGC

  

32kD α-antigen c

NTM F

TTCCTGACCAGCGAGCTGCCG

506

Ohara et al., 1993 [40]

 

NTM R

CCCCAGTACTCCCAGCTGTGC

  
  1. aspecific for Mycobacterium tuberculosis
  2. bspecific for Mycobacterium tuberculosis complex
  3. cspecific for non-tuberculous mycobacterium