Fig. 1

Evolutionary relationships of G. duodenalis isolated from selected test samples. The evolutionary history was inferred using the Neighbor-Joining method [44]. The optimal tree with the sum of branch length = 1.10022 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (2000 replicates) are shown next to the branches (Felsenstein et al., 1985). The evolutionary distances were computed using the p-distance method (Nei M et al., 2000) and are in the units of the number of base differences per site. The analysis involved 50 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 289 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 [20]. The samples are coded according to where they were recruited from and patient number. M1070 refers to Mukuru patient (outpatient) number 1070, MB108 refers to Mbagathi patient (inpatient) number 108