Fig. 2
From: Early detection of novel Leishmania species DNA in the saliva of two HIV-infected patients
PCR amplification of the ITS1 gene against saliva, buffy coat, blood, and tissue samples of nodular leishmaniasis case (a) and asymptomatic case (b). PCR amplicons were analyzed by electrophoresis on a 1.5 % agarose gel and stained with ethidium bromide. Lane S1, B1 and BF1: first saliva, blood and buffy coat collection, respectively; lane S2, B2 and BF2:second saliva, blood and buffy coat collection, respectively; and lane S3,B3 and BF3: third saliva, blood and buffy coat collection, respectively; T: tissue, lane M: molecular mass marker (100 basepairs [bp]); lane P: positive control containing extracted DNA from cultured L. martiniquensis-produced fragments of 379 bp, lane N: negative control (no DNA template: double-distilled water); lanes N1–N3: negative control (DNA template from non-infected saliva, blood, and buffy coat, respectively); and a PCR for template DNA control shown below (628 bp)