Table 3 Virucidal activity of the “final formulation” with 2 % urea and 2 % citric acid against test viruses of the German (values with Aqua bidest. and FCS) and European Guidelines (values with clean conditions)

BVDV

80 %

DVV/RKI

Aqua bidest.

≥4.63 ± 0.16

≥4.63 ± 0.16

n.d.

n.d.

BVDV

80 %

DVV/RKI

FCS

≥4.63 ± 0.16

≥4.63 ± 0.16

n.d.

n.d.

Vaccinia virus

80 %

DVV/RKI

Aqua bidest.

≥5.44 ± 0.19

≥5.44 ± 0.19

≥5.44 ± 0.19

n.d.

Vaccinia virus

80 %

DVV/RKI

FCS

≥4.94 ± 0.26

≥5.51 ± 0.18

≥5.51 ± 0.18

n.d.

PV

80 %

DVV/RKI

Aqua bidest.

n.d.

4.32 ± 0.41

≥6.13 ± 0.35

n.d.

PV

80 %

DVV/RKI

FCS

n.d.

3.13 ± 0.37

4.57 ± 0.37

n.d.

PV

80 %

EN 14476

Clean conditions

n.d.

≥5.32 ± 0.34

≥5.75 ± 0.30

n.d.

AdV

80 %

DVV/RKI

Aqua bidest.

n.d.

3.75 ± 0.31

≥4.50 ± 0.29

≥5.13 ± 0.33

AdV

80 %

DVV/RKI

FCS

n.d.

3.44 ± 0.00

4.31 ± 0.42

4.94 ± 0.40

AdV

80 %

EN 14476

Clean conditions

n.d.

4.19 ± 0.31

≥5.38 ± 0.25

n.d.

SV40

80 %

DVV/RKI

Aqua bidest.

n.d.

≥5.44 ± 0.27

≥5.44 ± 0.27

≥5.88 ± 0.29

SV40

80 %

DVV/RKI

FCS

n.d.

3.75 ± 0.55

4.32 ± 0.34

≥5.38 ± 0.25

MNV

80 %

EN 14476

Clean conditions

n.d.

4.13 ± 0.39

≥5.38 ± 0.29

≥5.88 ± 0.33