Table 1 Overview describing German, European and North Americans norms for virucidal testing

Minimum spectrum of test organisms needed to claim activity against all enveloped viruses (limited spectrum virucidal activity)

BVDV,

vaccinia virus

vaccinia virus

murine norovirus,

adenovirus

not defined

not defined

not defined

not defined

not defined

Minimum spectrum of test organisms needed to claim activity against all viruses

(virucidal activity)

murine norovirus,

adenovirus,

poliovirus,

polyomavirus, SV40

low level:

vaccinia virus,

adenovirus,

murine norovirus,

high level:

adenovirus,

murine norovirus,

murine parvovirus

murine norovirus,

adenovirus,

poliovirus

murine norovirus,

adenovirus

Minimum decimal log reduction needed

4

4

4

4

not defined in the method

not defined in the method

not defined in the method

not defined in the method

Interfering substances for clean conditions

none (Aqua bidest.)

0.3 g/l bovine serum albumin

0.3 g/l bovine serum albumin

0.3 g/l bovine serum albumin

-

-

-

-

Interfering substances for dirty conditions

10 % fetal calf serum (FCS)

3 g/l bovine serum albumin + 3 ml / l sheep erythrocytes

3 g/l bovine serum albumin + 3 ml / l sheep erythrocytes

3 g/l bovine serum albumin + 3 ml / l sheep erythrocytes

5 %

bovine serum

5 % bovine serum

5 % bovine serum

5 % bovine serum

Test concentration of rtu product

80 % or 90 %

100 %

80 % or 97 %

100 %

100 %

100 %

100 %