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Table 3 Total precision (variance, standard deviation and CV %) of PCR assays for HCV genotypes 2b, 1a, 3a and 6a (mean log10 HCV RNA viral load) at different key concentrations - within assay ranges

From: Traceability, reproducibility and clinical evaluation of Sansure Realtime HCV RNA assay

HCV genotype Expected HCV RNA (IU/ml) Expected HCV RNA (log10 IU/ml) No. tests performed Observed mean HCV RNA (log10 IU/ml) Difference of observed and expected HCV RNA (log10 IU/ml) Total precision variance (SD) of log10 HCV RNA Lognormal CV%
WWHV303-02 (1b) 25 1.398 9 1.372 −0.026 0.019 (0.137) 10.01
  50 1.699 9 1.665 −0.034 0.004 (0.067) 4.023
  100 2 9 1.951 −0.049 0.008 (0.088) 4.486
  1000 3 9 2.959 −0.041 0.022 (0.150) 5.055
  10000 4 9 4.015 0.015 0.003 (0.057) 1.410
  100000 5 9 5.049 0.049 0.002 (0.050) 0.989
WWHV303-04 (2a) 25 1.398 9 1.411 0.013 0.012 (0.109) 7.755
  50 1.699 9 1.710 0.011 0.009 (0.093) 5.453
  100 2 9 2.022 0.022 0.016 (0.126) 6.231
  1000 3 9 2.886 −0.114 0.017 (0.130) 4.513
WWHV303-11 (3a) 25 1.398 9 1.379 −0.019 0.013 (0.113) 8.181
  50 1.699 9 1.737 0.038 0.007 (0.080) 4.664
  100 2 9 2.044 0.044 0.019 (0.139) 6.798
  1000 3 9 2.893 −0.107 0.023 (0.150) 5.185
WWHV303-19 (6a) 25 1.398 9 1.413 0.015 0.013 (0.112) 7.958
  50 1.699 9 1.712 0.013 0.008 (0.087) 5.100
  100 2 9 1.980 −0.02 0.026 (0.162) 8.170
  1000 3 9 2.915 −0.085 0.017 (0.130) 4.478
  1. Abbreviations: SD standard deviation