Diarrhoeagenic Escherichia coli in mother-child Pairs in Ile-Ife, South Western Nigeria

BMC Infectious Diseases

Table 1 Polymerase chain reaction primers for diarrhoeagenic genes, IS3 and fliC of Escherichia coli ^a

Target gene or probe	Type	Primer Designation	Primers (5’ to 3’)	Amplicon size (base pair)
eae	EPEC/EHEC	eae 1	CTGAACGGCGATTACGCGAA	917
		eae 2	CCAGACGATACGATCCAG
bfp	EPEC	bfp 1	AATGGGCTTGCGCTTCCAG	326
		bfp 2	GCCGCTTTATCCAACCTGGTA
CVD432	EAEC	EAEC1	CTGGCGAAAGACTGTATCAT	630
		EAEC2	CAATGTATAGAAATCCGCTGTT
LT gene	ETEC	LTf	GGCGACAGATTATACCGTGC	450
		LTr	CGGTCTCTATTATACCGTGC
ST gene	ETEC	STf	ATTTTTMTTTCTGTATTRTCTT	190
		STr	CACCCGGTACARGCAGGATT
ipaH	EIEC	IpaH1	GTTCCTTGACCGCCTTTCCGATACCGTC	600
		IpaH2	GCCGGTCAGCCACCCTCTGAGAGTAC
Stx1	EHEC/STEC	Stx1f	ATAAATCGCCATTCGTTGACTAC	180
		Stx2r	AGAACGCCCACTGAGATCATCC
stx2	EHEC/STEC	Stx2f	GGCACTGTCTGAAACTGCTCC	255
		Stx2r	TCGCCAGTTATCTGACATTCTG
IS3	N/A	IS3	ACACTTAGCCGCGTGTCC	Variable
fliC	N/A	F-FLIC1	ATGGCACAAGTCATTAATACCCAAC	Variable
		R-FLIC2	CTAACCCTGCAGCAGAGACA

^a eae intimin, EPEC Enteropathogenic E. coli, EHEC Enterohemorrhagic E. coli, bfp bundle forming pilus, CVD432 aggregative adherence plasmid marker, EAEC Enteroaggregative E. coli, LT Heat-labile toxin, ETEC Enterotoxigenic E. coli, ST Heat-stable toxin, ipaH invasive plasmid antigen, EIEC Enteroinvasive E. coli, stx shiga toxin, STEC shiga toxin producing E. coli, N/A Not applicable