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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: Complex interaction between dengue virus replication and expression of miRNA-133a

Fig. 4

DENV up-regulates the expression of PTB through negative regulation of miRNA-133a. Vero cells were transfected with the synthetic form of miRNA-133a at a final concentration of 50 μM or 100 μM, and 24 h post-transfection PTB expression was assessed by Western blotting (a). Vero cells were challenged with DENV-2 NGC with a MOI of 3. PTB expression was measured by Western blotting at 12, 24 and 48 hpi (b). Finally, Vero cells were transfected with the synthetic form of miRNA-133a at a final concentration of 100 μM, and 24 hpt were challenged with DENV-2 at a MOI of 3. PTB expression was then measured by western blotting at 12, 24, 48 and 72 h (d). For (a, b), tubulin was used as loading control; for (d), actin was used as loading control. Semi-quantitative data of the western blot was assessed using Image J (c, e)

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