Fig. 3

miRNA-133a suppresses DENV-2 replication. Vero cells were transfected with the synthetic form of miRNA-133a or with the miRNA-133a inhibitor as mimic, both at a final concentration of 50 μM. At 24 hpt, cells were challenged with DENV-2 at a MOI of 3 and infection was evaluated at 12, 24, 48 and 72 hpi. a Flow cytometry of DENV-2 challenged cells, miRNA-133a transfected and infected cells, and miRNA inhibitor transfected and infected cells. b Viral RNA copy number of DENV-2 infected cells, miRNA-133a transfected and infected cells, and miRNA inhibitor transfected and infected cells assessed by RT-PCR in culture supernatants. c Viral titer of DENV-2 infected cells, miRNA-133a transfected and infected cells, and miRNA inhibitor transfected and infected cells assessed by flow cytometry. Data are shown as median ± range from three repeated experiments. (*) Statistically significant difference compared to the control (p < 0.05)