Fig. 2

MiRNA-133a alters the expression of GFP-fused to the DENV RNA 3'UTR. Vero cells were co-transfected with pGUD1, pGUD2, pGUD4 or synthetic miRNA-133a mimics and 24 h later, the expression of GFP was assessed by fluorescence microscopy (a) and western blot (b). Actin was used as a loading control. Semi-quantitative data of the western blots were assessed using Image J (c). A representative experiment is shown. In total three experiments were performed