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Table 2 Comparison of anti-HEV IgM with in-house ELISA and Wantai ELISA

From: Hepatitis E virus seroprevalence in pregnant women in Jiangsu, China, and postpartum evolution during six years

Sample In-house ELISA Wantai ELISA
OD450 Resulta OD450 Resultb
Polypeptide Polypeptide    
459–607 472–607    
B024042 1.105 0.409 + 0.294 +
F430527 1.377 0.429 + 0.403 +
193A 1.142 0.437 + 0.309 +
315A 1.392 0.502 + 0.309 +
  1. aELISA microplates were separately coated with purified immunodominant recombinant ORF2 polypeptide 459–607, and the truncated polypeptide 472–607. The OD450 value of the polypeptide 459–607 was denoted as OD459–607, and that of the polypeptide 472–607 was as OD472–607. Samples which showed OD459–607 ≥ 0.5 and the ratio of OD459–607 and OD472–607 > 2 or had the difference of OD459–607 minus OD472–607 > 0.5 were considered to be positive for anti-HEV IgM
  2. bThe OD450 values of 0.263 or above were considered to be anti-HEV IgM positive according to the instruction of the Wantai ELISA