Fig. 3

a H&E stain of atypical epithelium, showing hyperchromatic nuclei and transformed nucleus-cytoplasm ratio. Most of the squamous epithelium is covered by endocervical secretory cells, fulfilling the diagnosis of metaplasia. Intraepithelial lymphocytes mimic mitoses. b p16 immunohistochemistry. Same area as shown in Fig. 3a, the squamous epithelium is p16 negative, thus indicating that this lesion is not related to an HPV high-risk infection. c Ki-67 immunohistochemistry. Same area as shown in Fig. 3a and b. Only very few basal and parabasal cells are marked dark brown (physiological area). Intraepithelial lymphocytes in the upper half are negative