Skip to main content

Table 1 Oligonucleotide primer sequences used for the detection of β-lactamases in four Multiplex PCR assays of A. baumannii clinical isolates

From: High prevalence of oxacillinases in clinical multidrug-resistant Acinetobacter baumannii isolates from the Tshwane region, South Africa – an update

Target

Primer name

Primer sequence (5’ to 3’)a

Amplicon size (bp)

Reference

Multiplex I:

 OXA-23

bla OXA-23 (F)

GATCGGATTGGAGAACCAGA

501

[51]

bla OXA-23 (R)

ATTTCTGACCGCATTTCCAT

 OXA-24

bla OXA-24 (F)

GGTTAGTTGGCCCCCTTAAA

246

bla OXA-24 (R)

AGTTGAGCGAAAAGGGGATT

 OXA-51

bla OXA-51 (F)

TAATGCTTTGATCGGCCTTG

353

bla OXA-51 (R)

TGGATTGCACTTCATCTTGG

 OXA-58

bla OXA-58 (F)

AAGTATTGGGGCTTGTGCTG

599

bla OXA-58 (R)

CCCCTCTGCGCTCTACATAC

Multiplex II:

 VIM

bla VIM (F)

GATGGTGTTTGGTCGCATA

390

[52]

bla VIM (R)

CGAATGCGCAGCACCAG

 KPC

bla KPC (F)

CATTCAAGGGCTTTCTTGCTGC

538

bla KPC (R)

ACGACGGCATAGTCATTTGC

 IMP

bla IMP (F)

TTGACACTCCATTTACDGb

139

bla IMP (R)

GATYGAGAATTAAGCCACYCT

Multiplex III:

 GES

bla GES (F)

AGTCGGCTAGACCGGAAAG

399

[52]

bla GES (R)

TTTGTCCGTGCTCAGGAT

 PER

bla PER (F)

GCTCCGATAATGAAAGCGT

520

bla PER (R)

TTCGGCTTGACTCGGCTGA

 VEB

bla VEB (F)

CATTTCCCGATGCAAAGCGT

648

bla VEB (R)

CGAAGTTTCTTTGGACTCTG

Multiplex IV:

 GIM

bla GIM (F)

CGTTGCCAGCTTTAGCTCAGG

279

[53]

bla GIM (R)

GCAACTTGATACCAGCAGTGCG

 SPM

bla SPM (F)

GGGTGGCTAAGACTATGAAGCC

447

bla SPM (R)

GCCGCCGAGCTGAATCGG

 SIM-1

bla SIM (F)

TTGCGGAAGAAGCCCAGCCAG

613

bla SIM (R)

GCGTCTCCGATTTCACTGTGGC

 NDM – 1

bla NDM-1(F)

CCCGGCCACACCAGTGACA

129

bla NDM-1(R)

GTAGTGCTCAGTGTCGGCAT

  1. aAll oligonucleotides were synthesised and purified by Inqaba Biotechnical Industries, South Africa
  2. bY = T or C; D = A or G or T