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Table 1 Comparison of operational characteristics the three molecular assays

From: Performance evaluation of three commercial molecular assays for the detection of Mycobacterium tuberculosis from clinical specimens in a high TB-HIV-burden setting

  Anyplex™ plus MTB/NTM/DR-TB GenoType MTBDRplus version 2.0 Xpert® MTB/RIF
Principle Two-step multiplex real time PCR Reverse hybridisation Hemi-nested real-time PCR
End result MTBC, NTM, RIF, INH MTBC, RIF, INH MTBC, RIF
Level of complexity High High Low
Turn-around-time (including DNA extraction) 3.5 h 5.35 h 2 h
Result interpretation Automated Manuala Automated
Throughput per run 96 48 16b
Laboratory infrastructure Open system requiring three separate work areas. Open system requiring three separate work areas. Closed system, bench top within a temperature controlled area
  1. aGenoscan required for automated interpretation of results but at an additional cost
  2. bDepends on which module you have (e.g. 4, 16, or 48)