One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples

BMC Infectious Diseases

Table 3 Comparable analysis between the serotype-specific RT-PCR assays and the CDC DENV1-4 RT-PCR

A sample was classified as positive in the CDC DENV-1-4 real-time RT-PCR assays if the amplification curve crossed the threshold line within 37 cycles
Five of the six DENV1 RT-PCR positive samples, two of the six DENV2 RT-PCR positive samples, both the DENV3 RT-PCR positive samples, and the DENV4 RT-PCR positive sample that tested negative in respective CDC DENV-1-4 RT-PCR singleplex assay crossed the threshold line between 37 and 40 cycles in the CDC assays

ISSN: 1471-2334