One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples

BMC Infectious Diseases

Table 2 Specificity of the DENV1, DENV2, DENV3, and DENV4 real-time RT-PCR assays

Sample	No. of positive samples/no. tested
	DENV1 RT-PCR	DENV2 RT-PCR	DENV3 RT-PCR	DENV4 RT-PCR
DENV1 isolates	11/11	0/11	0/11	0/11
DENV2 isolates	0/5	5/5	0/5	0/5
DENV3 isolates	0/6	0/6	6/6	0/6
DENV4 isolates	0/2	0/2	0/2	2/2
QCMD panel, DENV1	17/17	0/17	0/17	0/17
QCMD panel, DENV2	0/11	11/11	0/11	0/11
QCMD panel, DENV3	0/8	0/8	8/8	0/8
QCMD panel, DENV4	0/4	0/4	0/4	4/4
QCMD negative controls	0/8	0/8	0/8	0/8

RNA preparations from DENV1, DENV2, DENV3, and DENV4 isolates obtained Cq-values between 16 and 25 when analyzed using their respective serotype-specific RT-PCR assay
The concentrations of DENV1, DENV2, and DENV3 included in the QCMD panels were 10³ to 10⁶, 10² to 10⁵, and 10³ to 10⁵ copies/mL, respectively. The concentration of DENV4 was 10⁵ copies/mL in all four QCMD panel samples

ISSN: 1471-2334