Fig. 2
From: One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples
Limit of detection. Limit of detection was determined by assaying eight replicates of twofold serially diluted RNA transcripts in three separate experiments. The number of positives per total number of replicates tested is shown for (a) the DENV1 RT-PCR assay, (b) the DENV2 RT-PCR assay, (c) the DENV3 RT-PCR assay, and (d) the DENV4 RT-PCR assay. Horizontal lines indicate mean values, boxes denote the 25th to 75th percentiles, whiskers the 5–95 % percentiles, and dots represent outliers