Skip to main content

Table 4 Analysis of clinical samples. RPA and PCR assay reactivity

From: Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood

Sample IDa

RPA / PCRb

 

1 μL

5 μL

BS 12/2013/1

+

 

BS 12/2013/2

+

 

KH 12/2013/1

-

+

KH 12/2013/2

+

 

DK 10/2011/2

+

 

MG 03/2012

+

 

EM 03/2012

-

+

MF 03/2012

-

+

AF 02/2012/1

+

 

AF 02/2012/2

+

 

MR 04/2013/2

+

 

HC1

 

-

HC2

 

-

HC3

 

-

HC4

 

-

  1. aSamples HC1, HC2, HC3 and HC4 were obtained from healthy individuals
  2. b1 or 5 μL of purified DNA was required to produce a positive signal in the RPA or PCR