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Table 3 Analytical sensitivity

From: Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood

Input Level

# of Replicates Tested

# of Replicates Detected

# of Replicates Detected

Stress (GE)

 

RPA

PCR

8

12

12

12

7

12

12

12

6

12

12

12

5

12

12

11

4

12

12

11

3

12

9

8

2

12

8

9

1

12

8

4

  1. The number of replicates producing positive signals following RPA and PCR amplification of varying (8, 7, 6, 5, 4, 3, 2, or 1) S. pneumoniae genome equivalents (GE)