Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood

Table 2 Nucleic Acid sequences of primers and probes

Probe/primer	DNA sequence (5’-3’)	Nucleotide position^a	Amplicon size (bp)
RPA			190
Forward	ACAGCTCCGTCTGTTATTTACAAAGTTAATTTGA*C	1123–1157
Reverse	AGTCCCCACGCTTACGCTGAGCTAGCTCCATTAC*T	1278–1312
Probe	CTTGACATAAGGCTCTTCAATGGTCGCAATCT[T(FAM)]A(dSpacer)[T(BHQ-1)]TGGGTCTGGAAAC*T	1193–1242
PCR			107
Forward	CTCGTAAGCGTAAACTCCTTG	1706–1727
Reverse	CATACTCAAGACGCTGAGGA	1793–1813
Probe	FAM-ACGCATGAAATCCATCGGATCAGTT-TAMRA	1749–1773

^aThe nucleotide position refers to the LepA gene from S. pneumoniae strain SPNA45 (Genbank accession number NC_018594.1)
*indicates a phosphorothioate bond

ISSN: 1471-2334