Fig. 3From: Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole bloodRPA assay robustness. The graphs show the fluorescence intensity obtained from the amplification of 50, 20 and 4 genome equivalents (GE) of S. pneumoniae (type strain; DSM20566), a in the absence of background human genomic DNA, b in the presence of 200Â ng background human genomic DNA and c in the presence of 400Â ng background human genomic DNA. Also shown in each graph is the fluorescence intensity obtained from the amplification of 100 GE (positive control, PC) of S. pneumoniae (type strain; DSM20566) and a no template control reaction (NTC)Back to article page