Phylogenetic relationship of HEV sequences derived from wild boars, Wetteraukreis district, Central Germany, 2013. The sequences were selected based on a sequence similarity search using the BLASTn search facility and additional human, pig and wild boar HEV reference strains (in italics) were included; rat HEV was used as an outgroup sequence. The strain designation, source of the viruses (human, pig, wild boar; liver, muscle), year of detection, country/region (GER – Germany), and the GenBank accession numbers are indicated if available. Grouping of the sequences into genotypes is shown on the right. The tree was constructed with a 148 nucleotide fragment of the HEV ORF2 using the neighbour-joining method implemented in the MEGALIGN module of the DNASTAR software package (Lasergene). Bootstrap values >50 % are indicated. HEV sequences derived from wild boars from this study are indicated in boldface and marked with an arrow.