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Table 1 Antigens and conditions used in each assay

From: Microsphere-based antibody assays for human parvovirus B19V, CMV and T. gondii

SIAs Antigen (Ag) Ag amount
(/coupling)
Serum
dilution
Cutoff
criterion
Cutoff
B19V VP2 IgG Recombinant VP2 50 μg 1:20 Mean + 4 SD Negative < 453 MFIa
Mean + 5 SD Positive > 532 MFIa
CMV IgG Purified virion lysate 20 μg 1:20 Mean + 4 SD Negative < 695 MFIa
Mean + 5 SD Positive > 834 MFIa
T. gondii IgG Purified lysate 50 μg 1:20 Mean + 4 SD Negative < 234 MFIa
Positive > 234 MFIa
  1. aMFI, median fluorescence intensity