Microsphere-based antibody assays for human parvovirus B19V, CMV and T. gondii

BMC Infectious Diseases

Table 1 Antigens and conditions used in each assay

SIAs	Antigen (Ag)	Ag amount (/coupling)	Serum dilution	Cutoff criterion	Cutoff
B19V VP2 IgG	Recombinant VP2	50 μg	1:20	Mean + 4 SD	Negative < 453 MFI^a
B19V VP2 IgG	Recombinant VP2	50 μg	1:20	Mean + 5 SD	Positive > 532 MFI^a
CMV IgG	Purified virion lysate	20 μg	1:20	Mean + 4 SD	Negative < 695 MFI^a
CMV IgG	Purified virion lysate	20 μg	1:20	Mean + 5 SD	Positive > 834 MFI^a
T. gondii IgG	Purified lysate	50 μg	1:20	Mean + 4 SD	Negative < 234 MFI^a
T. gondii IgG	Purified lysate	50 μg	1:20	Mean + 4 SD	Positive > 234 MFI^a

ISSN: 1471-2334